Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique

Abstract

Objective: To vitrify mouse and human blastocysts with use of the cryoloop procedure and to assess subsequent development. Design: Controlled study of vitrification of mouse and human blastocysts. Setting: Research department of a private assisted reproductive technology unit. Patient(s): Blastocysts that were not suitable to be frozen were donated from patients. Intervention(s): Culture of pronucleate embryos in sequential media to the blastocyst stage. Main Outcome Measure(s): Survival of the vitrification procedure was assessed by reexpansion, hatching, and outgrowth in culture. In addition, the viability of mouse blastocysts was assessed after transfer to pseudopregnant recipients. Resul..