Journal article

Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique

M Lane, WB Schoolcraft, DK Gardner

FERTILITY AND STERILITY | ELSEVIER SCIENCE INC | Published : 1999

Abstract

OBJECTIVE: To vitrify mouse and human blastocysts with use of the cryoloop procedure and to assess subsequent development. DESIGN: Controlled study of vitrification of mouse and human blastocysts. SETTING: Research department of a private assisted reproductive technology unit. PATIENT(S): Blastocysts that were not suitable to be frozen were donated from patients. INTERVENTION(S): Culture of pronucleate embryos in sequential media to the blastocyst stage. MAIN OUTCOME MEASURE(S): Survival of the vitrification procedure was assessed by reexpansion, hatching, and outgrowth in culture. In addition, the viability of mouse blastocysts was assessed after transfer to pseudopregnant recipients. RESUL..

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University of Melbourne Researchers