Journal article

Rapid full-length genomic sequencing of two cytopathically heterogeneous Australian primary HIV-1 isolates.

RB Oelrichs, VA Lawson, KM Coates, C Chatfield, NJ Deacon, DA McPhee

J Biomed Sci | Published : 2000


Two Australian HIV-1 isolates, derived from patient blood (HIV(MBC200)) and cerebrospinal fluid (HIV(MBC925)), were characterized after in vitro culture in peripheral blood mononuclear cells (PBMC). Although virus replication was similar, as measured by cell-free reverse transcriptase activity, only one of the two isolates (HIV-1(MCB200)) consistently induced cell syncytia and depleted the PBMC population of CD4+ cells by cell killing. A novel technique, devised for rapidly obtaining high-quality viral sequence data and the full-length genomic sequence of these two isolates, is presented. Analysis of the predicted sequence of the viral Env proteins provides correlates of the observed phenoty..

View full abstract

University of Melbourne Researchers