Fab fragments of ovine antibody to colchicine enhance its clearance in the rat
Philip W Peake, Timothy J Pianta, Lena Succar, Mangalee Fernando, Nicholas A Buckley, Zoltan HE Ndre
CLINICAL TOXICOLOGY | TAYLOR & FRANCIS LTD | Published : 2015
CONTEXT: Colchicine is an anti-inflammatory alkaloid used for the treatment of acute gout, but has a narrow therapeutic index. Colchicine overdoses are relatively rare, but have high mortality requiring rapid treatment. OBJECTIVE: To evaluate the ability of a newly available ovine fragment antigen-binding (Fab) antibody to colchicine (ColchiFab(™)) to protect rats against renal and other injury 24 h after colchicine ingestion. MATERIALS AND METHODS: Rats were gavaged with colchicine (5 mg/kg), then 2 h later injected intraperitoneally with 5 ml of sterile saline, or Fab anti-colchicine, a newly available ovine antibody to colchicine. Samples of blood were taken at 1, 2, 5 and 24 h after gava..View full abstract
Awarded by National Health and Medical Research grant
Fab anti-colchicine was a gift from Micropharm Ltd. without conditions as to its use or the publishing of data on its clinical effectiveness. Partial funding was provided by a National Health and Medical Research grant 1011772. The authors report no other interests.