Journal article
Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes
PK Perera, RB Gasser, DJ Pulford, MA Stevenson, SM Firestone, AMJ McFadden, A Jabbar
Parasites and Vectors | BIOMED CENTRAL LTD | Published : 2015
Open access
Abstract
Background: Oriental theileriosis is a tick-borne disease of bovines caused by the members of the Theileria orientalis complex. Recently, we developed a multiplexed tandem (MT) PCR to detect, differentiate and quantitate four genotypes (i.e., buffeli, chitose, ikeda and type 5) of T. orientalis. In this study, we used MT PCR to assess the prevalence and infection intensity of four T. orientalis genotypes in selected cattle herds that experienced oriental theileriosis outbreaks in New Zealand, and compared the sensitivities and specificities of MT PCR, PCR-high resolution melting (PCR-HRM) and a TaqMan® qPCR. Methods: MT PCR, PCR-HRM analysis for T. orientalis and a TaqMan® qPCR assay for ike..
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
This project was partially supported by the Department of Agriculture Fisheries and Forestry (DAFF), a Collaborative Research Grant (the University of Melbourne) (A.J.) and the Australian Research Council (ARC) (R.B.G. et al.). Funding from the CASS Foundation and The Ian Potter Foundation is also gratefully acknowledged (A.J.). P.P. is a grateful recipient of the International Postgraduate Research Scholarship (IPRS) and Australian Postgraduate Award (APA) through The University of Melbourne. We thank Dr Aaron R. Jex for allowing the use the Easy-Plex platform (AusDiagnostics Pty. Ltd., Australia), which was bought under project 1043, funded by Water Quality Research Australia, and contributions from the Melbourne Water Corporation. We thank all of the veterinary practitioners and dairy farmers from New Zealand, who assisted by collecting blood samples, and the staff in Virology at the Animal Health Laboratory Wallaceville, particularly Dr Edna Gias, for technical support. We are grateful for Dr Munir Aktas from University of Firat, Turkey, for the provision of some parasite material.