Journal article

A serine-arginine-rich (SR) splicing factor modulates alternative splicing of over a thousand genes in Toxoplasma gondii

Lee M Yeoh, Christopher D Goodman, Nathan E Hall, Giel G van Dooren, Geoffrey I McFadden, Stuart A Ralph

NUCLEIC ACIDS RESEARCH | OXFORD UNIV PRESS | Published : 2015

Abstract

Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoured in this phylum. We investigated the mechanism of alternative splicing in Toxoplasma gondii, and have identified and localized TgSR3, a homologue of ASF/SF2 (alternative-splicing factor/splicing factor 2, a serine-arginine-rich, or SR protein) to a subnuclear compartment. In addition, we conditionally overexpressed this protein, which was deleterious to growth. qRT-PCR was used..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by NHMRC


Funding Acknowledgements

Australian Postgraduate Award [to L.M.Y.]; Australian Research Council (ARC) QEII fellowship [DP110103144 to G.G.v.D.]; National Health and Medical Research Council of Australia (NHMRC) Program Grant [DP130103236 to G.I.M.]; ARC Project Grant [637406 to G.I.M.]; NHMRC RDWright Biomedical Fellowship [APP1062504 to S.A.R.]. Funding for open access charge: Australian National Health and Medical Research Council grants.