Differences in the resting platelet proteome and platelet releasate between healthy children and adults

C Cini; C Yip; C Attard; V Karlaftis; P Monagle; M Linden; V Ignjatovic

Journal article

Differences in the resting platelet proteome and platelet releasate between healthy children and adults

C Cini, C Yip, C Attard, V Karlaftis, P Monagle, M Linden, V Ignjatovic

Journal of Proteomics | ELSEVIER | Published : 2015

DOI: 10.1016/j.jprot.2015.04.003

Abstract

Major age-related diseases such as cardiovascular disease and cancer are the primary causes of morbidity and mortality in Australia and worldwide. In our recent study characterising differences in the plasma proteome between healthy children and adults, a large number of proteins differentially expressed with age were found to be of platelet origin. This study aimed to characterise differences in the resting platelet proteome and the platelet releasate of healthy children compared to healthy adults. This study represents the setup of a procedure for the proteomic analysis of platelets from children. Differentially expressed platelet proteins were identified using Two-dimensional Differential..

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University of Melbourne Researchers

Vicky Karlaftis Author

Paul Monagle Author

Vera Ignjatovic Author

Grants

Funding Acknowledgements

The authors would like to thank the Anaesthetic and Surgical departments of the Royal Children's Hospital, Melbourne for facilitating the sample collection; X and Y from the Mass Spectrometry Facility at Bio21, and The University of Melbourne for their technical assistance. We would also like to thank all participants. This study was performed with the support of the Murdoch Childrens Research Institute Clinical Science theme funding. This study was supported by the Victorian Government's Operational Infrastructure Support Program.