Journal article
Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells
W Shi, Y Liao, SN Willis, N Taubenheim, M Inouye, DM Tarlinton, GK Smyth, PD Hodgkin, SL Nutt, LM Corcoran
Nature Immunology | Published : 2015
DOI: 10.1038/ni.3154
Abstract
When B cells encounter an antigen, they alter their physiological state and anatomical localization and initiate a differentiation process that ultimately produces antibody-secreting cells (ASCs). We have defined the transcriptomes of many mature B cell populations and stages of plasma cell differentiation in mice. We provide a molecular signature of ASCs that highlights the stark transcriptional divide between B cells and plasma cells and enables the demarcation of ASCs on the basis of location and maturity. Changes in gene expression correlated with cell-division history and the acquisition of permissive histone modifications, and they included many regulators that had not been previously ..
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Awarded by Multiple Myeloma Research Foundation
Funding Acknowledgements
We thank D. Emslie, T. Kratina, M. Everest and S. Heinzel for technical assistance and J. Vasiliadis, J. Leahy and T. Camilleri for animal care. Socs2<SUP>-/-</SUP> mice were a gift from W. Alexander (The Walter and Eliza Hall Institute, Parkville, Victoria, Australia). The institutional flow cytometry facility provided essential services. Supported by the National Health and Medical Research Council (NHMRC) of Australia (NHMRC IRIISS grant 361646; Program Grants 575500 and 1054925 to D. M. T., P.D.H., S.L.N. and L.M.C.; Program Grant 1054618 to G.K.S.; Project Grant 1023454 to G.K.S. and W. S.; and fellowships to G.K.S., D. M. T., P.D.H. and L.M.C.), the Multiple Myeloma Research Foundation (Senior Award to S.L.N.), the Australian Research Council (ARC Future Fellowship to S.L.N.) and the Victorian State Government through an Operational Infrastructure Support grant.