Standard flow liquid chromatography for shotgun proteomics in bioenergy research
Susana M Gonzalez Fernandez-Nino, A Michelle Smith-Moritz, Leanne Jade G Chan, Paul D Adams, Joshua L Heazlewood, Christopher J Petzold
Frontiers in Bioengineering and Biotechnology | FRONTIERS MEDIA SA | Published : 2015
Over the past 10 years, the bioenergy field has realized significant achievements that have encouraged many follow on efforts centered on biosynthetic production of fuel-like compounds. Key to the success of these efforts has been transformational developments in feedstock characterization and metabolic engineering of biofuel-producing microbes. Lagging far behind these advancements are analytical methods to characterize and quantify systems of interest to the bioenergy field. In particular, the utilization of proteomics, while valuable for identifying novel enzymes and diagnosing problems associated with biofuel-producing microbes, is limited by a lack of robustness and limited throughput. ..View full abstract
Awarded by U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research
This work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U.S. Department of Energy.