Application of dynamic metabolomics to examine in vivo skeletal muscle glucose metabolism in the chronically high-fat fed mouse
Greg M Kowalski, David P De Souza, Micah L Burch, Steven Hamley, Joachim Kloehn, Ahrathy Selathurai, Dedreia Tull, Sean O'Callaghan, Malcolm J McConville, Clinton R Bruce
Biochemical and Biophysical Research Communications | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2015
RATIONALE: Defects in muscle glucose metabolism are linked to type 2 diabetes. Mechanistic studies examining these defects rely on the use of high fat-fed rodent models and typically involve the determination of muscle glucose uptake under insulin-stimulated conditions. While insightful, they do not necessarily reflect the physiology of the postprandial state. In addition, most studies do not examine aspects of glucose metabolism beyond the uptake process. Here we present an approach to study rodent muscle glucose and intermediary metabolism under the dynamic and physiologically relevant setting of the oral glucose tolerance test (OGTT). METHODS AND RESULTS: In vivo muscle glucose and interm..View full abstract
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Awarded by National Health and Medical Research Council
This work was supported by a grant from the Diabetes Australia Research Trust. MJM is supported by a fellowship from the National Health and Medical Research Council (APP1059530).