Journal article
Identification of antibody glycosylation structures that predict monoclonal antibody FC-effector function
AW Chung, M Crispin, L Pritchard, H Robinson, MK Gorny, X Yu, C Bailey-Kellogg, ME Ackerman, C Scanlan, S Zolla-Pazner, G Alter
AIDS | Published : 2014
Abstract
OBJECTIVE: To determine monoclonal antibody (mAb) features that predict Fc-mediated effector functions against HIV. DESIGN: Monoclonal antibodies, derived from Chinese hamster ovary cells or EBV-immortalized mouse heteromyelomas, with specificity to key regions of the HIV envelope including gp120-V2, gp120-V3 loop, gp120-CD4 binding site, and gp41-specific antibodies, were functionally profiled to determine the relative contribution of the variable and constant domain features of the antibodies in driving robust Fc-effector functions. METHODS: Each mAb was assayed for antibody-binding affinity to gp140, antibody -dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosi..
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Awarded by National Institutes of Health
Funding Acknowledgements
These studies were supported in part by Collaboration for AIDS Vaccine Discovery (CAVD) (OPP1032817: Leveraging Antibody Effector Function) (A. W. C., M. C., L. P., H. R., C. B. K., M. E. A., C. S., G. A.), National Health and Medical Research Council (NHMRC) APP1036470 (A. W. C.), HL59725 (S.Z.P.) and P01 AI100151 (S.Z.P.), and by research funds from the Department of Veterans Affairs.