Journal article

The C-terminal end of the capsid protein of Avian Nephritis Virus is antigenic and induces broadly cross-reactive antibodies

KA Hewson, NK Wawegama, D O'Rourke, K Putri, A Chamings, AH Noormohammadi, J Ignjatovic

Journal of Virological Methods | Elsevier | Published : 2015

Abstract

© 2015 Elsevier B.V. Avian nephritis virus (ANV) has been isolated frequently from commercial broilers in many countries. The prevalence and economic impact of ANV however has been difficult to ascertain due to the lack of convenient serological techniques. In this study the full-length and fragments of the ANV capsid protein were expressed in Baculovirus and affinity purified recombinant proteins used for the detection of ANV antibodies in ELISA. The crystal structure of Human Astrovirus (HAstV) was used as a model to determine potential homologous C-terminal antigenic regions in ANV. The rp37 fragment from three ANV strains NSW_3, ANV-1 and ANV-2, and a shorter NSW_3 fragment (rp33) were c..

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Funding Acknowledgements

The authors would like to thank Cheryl Colson, June Daly and Jenece Wheeler for their technical assistance, and Dr. Ben Wells, Dr. Soy Rubite and Dr. John Tchang for providing clinical specimens. Special thanks are due to Dr. Annette Bolte-Terberger, Lohmann Tierzucht Veterinary Laboratory, Cuxhaven, Germany for the testing provided. This investigation was supported in part with funding obtained from the Australian Rural Industries Research and Development Corporation (RIRDC).