Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma

AA Albarhani; F Collier; RF Greaves; AL Ponsonby; KJ Allen; PJ Vuillermin; P Roche; MW Clarke; D Burgner; J Carlin; T Dwyer; S Ranganathan; R Saffery; M Tang

Journal article

Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma

AA Albarhani, F Collier, RF Greaves, AL Ponsonby, KJ Allen, PJ Vuillermin, P Roche, MW Clarke, D Burgner, J Carlin, T Dwyer, S Ranganathan, R Saffery, M Tang

Clinical Biochemistry | Published : 2015

DOI: 10.1016/j.clinbiochem.2015.04.014

Abstract

Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a me..

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