Journal article
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
S Keerthikumar, L Gangoda, M Liem, P Fonseka, I Atukorala, C Ozcitti, A Mechler, CG Adda, CS Ang, S Mathivanan
Oncotarget | Published : 2015
Abstract
Extracellular vesicles (EVs) include the exosomes (30-100 nm) that are produced through the endocytic pathway via the multivesicular bodies and the ectosomes (100- 1000 nm) that are released through the budding of the plasma membrane. Despite the differences in the mode of biogenesis and size, reliable markers that can distinguish between exosomes and ectosomes are non-existent. Moreover, the precise functional differences between exosomes and ectosomes remains poorly characterised. Here, using label-free quantitative proteomics, we highlight proteins that could be exploited as markers to discriminate between exosomes and ectosomes. For the first time, a global proteogenomics analysis unveil..
View full abstractGrants
Awarded by Australian NHMRC fellowship
Awarded by Australian Research Council Discovery project grant
Funding Acknowledgements
SM is supported by the Australian NH&MRC fellowship (1016599), Australian Research Council Discovery project grant (DP130100535), Australian Research Council DECRA (DE150101777) and Ramaciotti Establishment grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors like to thank Hina Kalra for her help in the isolation of 10K pellet for LIM1215 colorectal cancer cells.