Journal article

MOZ and BMI1 play opposing roles during Hox gene activation in ES cells and in body segment identity specification in vivo

Bilal N Sheikh, Natalie L Downer, Belinda Phipson, Hannah K Vanyai, Andrew J Kueh, Davis J McCarthy, Gordon K Smyth, Tim Thomas, Anne K Voss

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2015

Abstract

Hox genes underlie the specification of body segment identity in the anterior-posterior axis. They are activated during gastrulation and undergo a dynamic shift from a transcriptionally repressed to an active chromatin state in a sequence that reflects their chromosomal location. Nevertheless, the precise role of chromatin modifying complexes during the initial activation phase remains unclear. In the current study, we examined the role of chromatin regulators during Hox gene activation. Using embryonic stem cell lines lacking the transcriptional activator MOZ and the polycomb-family repressor BMI1, we showed that MOZ and BMI1, respectively, promoted and repressed Hox genes during the shift ..

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Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by NHMRC


Funding Acknowledgements

We thank M. van Lohuizen for the Bmi1 mutant mice and are grateful to R. Cobb, C. Gatt, and F. Dabrowski for excellent technical support. This work was funded by Australian National Health and Medical Research Council (NHMRC) Project Grants 1010851, 1008699, 1030704 and 1051078, NHMRC Research Fellowships [1058892 (to G.K.S.), 1003435 (to T.T.), and 575512 and 1081421 (to A.K.V.)], NHMRC Dora Lush Scholarship (BNS), Australian Postgraduate Award (to H.K.V.), International Postgraduate Research Scholarship (A.J.K.), NHMRC Independent Research Institutes Infrastructure Support (IRIIS) Scheme and a Victorian State Government Operational Infrastructure Support (OIS) Grant.