The androgen receptor has no direct antiresorptive actions in mouse osteoclasts
Mieke Sinnesael, Ferran Jardi, Ludo Deboel, Michael R Laurent, Vanessa Dubois, Jeffrey D Zajac, Rachel A Davey, Geert Carmeliet, Frank Claessens, Dirk Vanderschueren
MOLECULAR AND CELLULAR ENDOCRINOLOGY | ELSEVIER IRELAND LTD | Published : 2015
Androgen deficiency or androgen receptor knockout (ARKO) causes high-turnover osteopenia, but the target cells for this effect remain unclear. To examine whether AR in osteoclasts directly suppresses bone resorption, we crossed AR-floxed with cathepsin K-Cre mice. Osteoclast-specific ARKO (ocl-ARKO) mice showed no changes neither in osteoclast surface nor in bone microarchitecture nor in the response to orchidectomy and androgen replacement, indicating that the AR in osteoclasts is not critical for bone maintenance. In line with the lack of a bone phenotype, the levels of AR were very low in osteoclast-enriched cultures derived from bone marrow (BM) and undetectable in osteoclasts generated ..View full abstract
Awarded by KU Leuven
Awarded by Research Foundation Flanders (FWO)
This study was funded by grant OT/09/035 and GOA/15/017 from KU Leuven and grant number G.0858.11 from the Research Foundation Flanders (FWO). M.R.L. and V.D. are PhD fellows of the Research Foundation Flanders (FWO). D.V. is senior investigator of the University Hospitals Leuven Clinical Research Fund.