Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
Marianna Marangi, Anson V Koehler, Sergio A Zanzani, Maria T Manfredi, Emanuele Brianti, Annunziata Giangaspero, Robin B Gasser
Parasites and Vectors | BMC | Published : 2015
BACKGROUND: Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. METHODS: A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. RESULTS: Cyclospora was detected in three Pan troglodytes (1..View full abstract
Awarded by Victorian Life Sciences Computation Initiative (VLSCI)
The authors thank Drs. Raffaella Terlizzi and Tiziana Caradonna for technical assistance. RBG's research is funded through the Australian Research Council (ARC), Melbourne Water Corporation, and the National Health and Medical Research Council (NHMRC) of Australia; it is also supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant number VR0007 on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government.