Pectin Biosynthesis: GALS1 in Arabidopsis thaliana Is a beta-1,4-Galactan beta-1,4-Galactosyltransferase
April Jennifer Madrid Liwanag, Berit Ebert, Yves Verhertbruggen, Emilie A Rennie, Carsten Rautengarten, Ai Oikawa, Mathias CF Andersen, Mads H Clausen, Henrik Vibe Scheller
The Plant Cell | OXFORD UNIV PRESS INC | Published : 2012
β-1,4-Galactans are abundant polysaccharides in plant cell walls, which are generally found as side chains of rhamnogalacturonan I. Rhamnogalacturonan I is a major component of pectin with a backbone of alternating rhamnose and galacturonic acid residues and side chains that include α-1,5-arabinans, β-1,4-galactans, and arabinogalactans. Many enzymes are required to synthesize pectin, but few have been identified. Pectin is most abundant in primary walls of expanding cells, but β-1,4-galactan is relatively abundant in secondary walls, especially in tension wood that forms in response to mechanical stress. We investigated enzymes in glycosyltransferase family GT92, which has three members in ..View full abstract
Awarded by Office of Science, Office of Biological and Environmental Research
This work conducted by the Joint BioEnergy Institute was supported by the Office of Science, Office of Biological and Environmental Research, through Contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U. S. Department of Energy and by the Danish Strategic Research Council. We thank Sherry Chan for assistance with plant growth.