Journal article

The relative population sizes of megakaryocytic cells in mouse bone marrow as determined by mpl ligand responsiveness

J Mintern, N Williams, H Jackson

EXPERIMENTAL HEMATOLOGY | CARDEN JENNINGS PUBL CO LTD | Published : 1997

Abstract

The relative population sizes of mpl ligand-responsive megakaryocytic cells were investigated, and all megakaryocytes grown in culture were assessed. Three groups were analyzed: 1) immature cells with the ability to form single mature megakaryocytes; 2) cells with the ability to divide once before forming megakaryocytes (doublets); and 3) progenitor cells with the ability to form colonies, i.e., to undergo both cytokinesis and maturation. Immature cells forming single megakaryocytes proved most sensitive to the mpl ligand. Committed megakaryocyte progenitors required approximately 30 times more mpl ligand to achieve maximum growth than did the immature megakaryocyte population. Similar numbe..

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