A De Novo Floral Transcriptome Reveals Clues into Phalaenopsis Orchid Flower Development
Jian-Zhi Huang, Chih-Peng Lin, Ting-Chi Cheng, Bill Chia-Han Chang, Shu-Yu Cheng, Yi-Wen Chen, Chen-Yu Lee, Shih-Wen Chin, Fure-Chyi Chen
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2015
Phalaenopsis has a zygomorphic floral structure, including three outer tepals, two lateral inner tepals and a highly modified inner median tepal called labellum or lip; however, the regulation of its organ development remains unelucidated. We generated RNA-seq reads with the Illumina platform for floral organs of the Phalaenopsis wild-type and peloric mutant with a lip-like petal. A total of 43,552 contigs were obtained after de novo assembly. We used differentially expressed gene profiling to compare the transcriptional changes in floral organs for both the wild-type and peloric mutant. Pair-wise comparison of sepals, petals and labellum between peloric mutant and its wild-type revealed 1,8..View full abstract
Awarded by Council of Agriculture, Agriculture and Food Agency, Taiwan
This work was supported by grants from the Council of Agriculture, Agriculture and Food Agency, Taiwan, with the grant numbers 103AS-9.1.1-FD-Z2 and 104AS-9.1.1-FD-Z2.