Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Zerina Lokmic, Elizabeth S Ng, Matthew Burton, Edouard G Stanley, Anthony J Penington, Andrew G Elefanty
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2015
Lymphatic system disorders such as primary lymphedema, lymphatic malformations and lymphatic tumors are rare conditions that cause significant morbidity but little is known about their biology. Isolating highly pure human lymphatic endothelial cells (LECs) from diseased and healthy tissue would facilitate studies of the lymphatic endothelium at genetic, molecular and cellular levels. It is anticipated that these investigations may reveal targets for new therapies that may change the clinical management of these conditions. A protocol describing the isolation of human foreskin LECs and lymphatic malformation lymphatic endothelial cells (LM LECs) is presented. To obtain a single cell suspensio..View full abstract
The authors would like to acknowledge the Baker Foundation and the Royal Children's Foundation 'Women in Science Fellowship' support of Zerina Lokmic. Andrew G. Elefanty and Edouard G. Stanley are NHMRC Senior Research Fellows. Work in their laboratories was supported by NHMRC and Stem Cells Australia.