Journal article

Investigating the first outbreak of oriental theileriosis in cattle in South Australia using multiplexed tandem PCR (MT-PCR)

Hagos Gebrekidan, Robin B Gasser, Piyumali K Perera, Sarah McGrath, Sean McGrath, Mark A Stevenson, Abdul Jabbar



This study investigated the first outbreak of oriental theileriosis in a herd of beef cattle in South Australia using a newly established multiplexed tandem PCR (MT-PCR) to identify, differentiate and quantitate the four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis recognised to occur in Australasia. Following clinical diagnosis of oriental theileriosis (based on clinical signs, laboratory findings and post mortem examination), 155 blood samples were collected from individual cows (n = 85) and calves (n = 70), and tested by MT-PCR. In total, 117 (75.48%) cattle were shown to be test-positive for T. orientalis. All four genotypes were detected, and ikeda had the high..

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Awarded by Water Quality Research Australia

Funding Acknowledgements

This project was partially supported by the Department of Agriculture Fisheries and Forestry (DAFF), a Collaborative Research Grant (the University of Melbourne) (A.J.) and the Australian Research Council (ARC) (R.B.G. et al.). Funding from the CASS Foundation and The Ian Potter Foundation is also gratefully acknowledged (A.J.). H.G. is a grateful recipient of the Melbourne International Research Scholarships (MIRS) and Melbourne International Fee Remission Scholarships (MIFRS) through The University of Melbourne. The Easy-Plex platform (AusDiagnostics) used in this project was bought under project 1043, funded by Water Quality Research Australia, and contributions from the Melbourne Water Corporation (Dr Aaron R. Jex). We thank the farmer for donating blood samples.