The Effect of an Alternate Start Codon on Heterologous Expression of a PhoA Fusion Protein in Mycoplasma gallisepticum
Indu S Panicker, Glenn F Browning, Philip F Markham
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2015
While the genomes of many Mycoplasma species have been sequenced, there are no collated data on translational start codon usage, and the effects of alternate start codons on gene expression have not been studied. Analysis of the annotated genomes found that ATG was the most prevalent translational start codon among Mycoplasma spp. However in Mycoplasma gallisepticum a GTG start codon is commonly used in the vlhA multigene family, which encodes a highly abundant, phase variable lipoprotein adhesin. Therefore, the effect of this alternate start codon on expression of a reporter PhoA lipoprotein was examined in M. gallisepticum. Mutation of the start codon from ATG to GTG resulted in a 2.5 fold..View full abstract
ISP was in receipt of an International Postgraduate Research Scholarship from the Commonwealth of Australia. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.