Journal article

Beyond the Western front: targeted proteomics and organelle abundance profiling

Harriet T Parsons, Joshua L Heazlewood

FRONTIERS IN PLANT SCIENCE | FRONTIERS MEDIA SA | Published : 2015

Abstract

The application of westerns or immunoblotting techniques for assessing the composition, dynamics, and purity of protein extracts from plant material has become common practice. While the approach is reproducible, can be readily applied and is generally considered robust, the field of plant science suffers from a lack of antibody variety against plant proteins. The development of approaches that employ mass spectrometry to enable both relative and absolute quantification of many hundreds of proteins in a single sample from a single analysis provides a mechanism to overcome the expensive impediment in having to develop antibodies in plant science. We consider it an opportune moment to consider..

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University of Melbourne Researchers

Grants

Awarded by U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research


Awarded by Australian Research Council Future Fellowship


Awarded by Marie Curie Intra European Fellowship


Funding Acknowledgements

This work was supported by the U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U. S. Department of Energy. JH is supported by an Australian Research Council Future Fellowship [FT130101165]. HP is supported by a Marie Curie Intra European Fellowship 2012 [FP7-PEOPLE-2011-IEF 301401].