Journal article
PtaRHE1, a Populus tremula × Populus alba RING-H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development
F Baldacci-Cresp, J Moussawi, JC Leplé, R Van Acker, A Kohler, J Candiracci, L Twyffels, AV Spokevicius, G Bossinger, F Laurans, N Brunel, M Vermeersch, W Boerjan, M El Jaziri, M Baucher
Plant Journal | Published : 2015
DOI: 10.1111/tpj.12867
Abstract
REALLY INTERESTING NEW GENE (RING) proteins play important roles in the regulation of many processes by recognizing target proteins for ubiquitination. Previously, we have shown that the expression of PtaRHE1, encoding a Populus tremula × Populus alba RING-H2 protein with E3 ubiquitin ligase activity, is associated with tissues undergoing secondary growth. To further elucidate the role of PtaRHE1 in vascular tissues, we have undertaken a reverse genetic analysis in poplar. Within stem secondary vascular tissues, PtaRHE1 and its corresponding protein are expressed predominantly in the phloem. The downregulation of PtaRHE1 in poplar by artificial miRNA triggers alterations in phloem fibre patt..
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Awarded by COST Action
Funding Acknowledgements
The authors thank Johnny Mukoko Bopopi for help with in situ mRNA hybridization, Veronique Olislagers for RNA quantification, and Maria Boczek and Nadege Millet for technical work. F.B. is a postdoctoral researcher of the FNRS-FRS in the frame of research project T.0004.14. J.M. is a scholar of the Lebanese National Council for Scientific Research and received support from the 'Fonds Alice et David Van Buuren'. M.B. is a senior research associate of the FRS-FNRS. R.V.A. and W.B. were supported by the UGent Multidisciplinary Research Partnership 'Biotechnology for a sustainable economy'. This study was supported by FRS-FNRS grant Credit aux chercheurs 1.5107.12, by the bilateral France-Wallonie-Bruxelles Tournesol programme and by COST Action FP0905. The CMMI is supported by the European Regional Development Fund and the Walloon Region. A.K. belongs to the Laboratory of Excellence ARBRE (Advanced Research on the Biology of Tree and Forest Ecosystems) (ANR-11-LABX-0002-01).