Journal article
Blood-Stage Parasitaemia and Age Determine Plasmodium falciparum and P-vivax Gametocytaemia in Papua New Guinea
Cristian Koepfli, Leanne J Robinson, Patricia Rarau, Mary Salib, Naomi Sambale, Rahel Wampfler, Inoni Betuela, Wang Nuitragool, Alyssa E Barry, Peter Siba, Ingrid Felger, Ivo Mueller
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2015
Abstract
A better understanding of human-to-mosquito transmission is crucial to control malaria. In order to assess factors associated with gametocyte carriage, 2083 samples were collected in a cross-sectional survey in Papua New Guinea. Plasmodium species were detected by light microscopy and qPCR and gametocytes by detection of pfs25 and pvs25 mRNA transcripts by reverse-transcriptase PCR (qRT-PCR). The parasite prevalence by PCR was 18.5% for Plasmodium falciparum and 13.0% for P. vivax. 52.5% of all infections were submicroscopic. Gametocytes were detected in 60% of P. falciparum-positive and 51% of P. vivax-positive samples. Each 10-fold increase in parasite density led to a 1.8-fold and 3.3-fol..
View full abstractGrants
Awarded by NHMRC project grant
Awarded by Swiss National Science Foundation grant
Awarded by NIH International Centers of Excellence in Malaria Research grant
Awarded by NHMRC Early Career Fellowship
Awarded by NHMRC Senior Research Fellowship
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
Funding Acknowledgements
This study was supported by the TransEPI consortium funded by the Bill & Melinda Gates Foundation (www.gatesfoundation.org), and an NHMRC project grant (#1021455, www.nhmrc.gov.au), Swiss National Science Foundation grant (310030_134889, www.snf.ch), and NIH International Centers of Excellence in Malaria Research grant (U19 AI089686, www.niaid.nih.gov). Samples were collected as part of the Bill & Melinda Gates Foundation funded PNG IPTi study. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. LJ Robinson is supported by an NHMRC Early Career Fellowship (#1016443). IM is supported by an NHMRC Senior Research Fellowship (#1043345). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.