Journal article
Blood-stage parasitaemia and age determine Plasmodium falciparum and P. vivax gametocytaemia in Papua New Guinea
C Koepfli, LJ Robinson, P Rarau, M Salib, N Sambale, R Wampfler, I Betuela, W Nuitragool, AE Barry, P Siba, I Felger, I Mueller
Plos One | Published : 2015
Abstract
A better understanding of human-to-mosquito transmission is crucial to control malaria. In order to assess factors associated with gametocyte carriage, 2083 samples were collected in a cross-sectional survey in Papua New Guinea. Plasmodium species were detected by light microscopy and qPCR and gametocytes by detection of pfs25 and pvs25mRNA transcripts by reverse-transcriptase PCR (qRT-PCR). The parasite prevalence by PCR was 18.5% for Plasmodium falciparumand 13.0% for P. vivax. 52.5% of all infections were submicroscopic. Gametocytes were detected in 60% of P. falciparum-positive and 51% of P. vivax-positive samples. Each 10-fold increase in parasite density led to a 1.8-fold and 3.3-fold ..
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Awarded by Bill and Melinda Gates Foundation
Funding Acknowledgements
This study was supported by the TransEPI consortium funded by the Bill & Melinda Gates Foundation (www.gatesfoundation.org), and an NHMRC project grant (#1021455, www.nhmrc.gov.au), Swiss National Science Foundation grant (310030_134889, www.snf.ch), and NIH International Centers of Excellence in Malaria Research grant (U19 AI089686, www.niaid.nih.gov). Samples were collected as part of the Bill & Melinda Gates Foundation funded PNG IPTi study. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. LJ Robinson is supported by an NHMRC Early Career Fellowship (#1016443). IM is supported by an NHMRC Senior Research Fellowship (#1043345). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.