Homozygous staggerer (sg/sg) mice display improved insulin sensitivity and enhanced glucose uptake in skeletal muscle
P Lau, RL Fitzsimmons, MA Pearen, MJ Watt, GEO Muscat
DIABETOLOGIA | SPRINGER | Published : 2011
AIMS/HYPOTHESIS: Homozygous staggerer (sg/sg) mice, which have decreased and dysfunctional Rorα (also known as Rora) expression in all tissues, display a lean and dyslipidaemic phenotype. They are also resistant to (high fat) diet-induced obesity. We explored whether retinoic acid receptor-related orphan receptor (ROR) α action in skeletal muscle was involved in the regulation of glucose metabolism. METHODS: We used a three-armed genomic approach, including expression profiling, ingenuity analysis and quantitative PCR validation to identify the signalling pathway(s) in skeletal muscle that are perturbed in sg/sg mice. Moreover, western analysis, functional insulin and glucose tolerance tests..View full abstract
G.E.O Muscat is a Principal Research Fellow and M.J. Watt a Senior Research Fellow of the National Health and Medical Research Council of Australia (NHMRC). This study was supported by project grants to G.E.O Muscat from the NHMRC and by Diabetes Australia Research Trust.