A tandem liquid chromatography-mass spectrometry (LC-MS) method for profiling small molecules in complex samples
James S Pyke, Damien L Callahan, Komal Kanojia, Jairus Bowne, Sheena Sahani, Dedreia Tull, Antony Bacic, Malcolm J McConville, Ute Roessner
METABOLOMICS | SPRINGER | Published : 2015
Liquid chromatography–mass spectrometry (LC–MS) methods using either aqueous normal phase (ANP) or reversed phase (RP) columns are routinely used in small molecule or metabolomic analyses. These stationary phases enable chromatographic fractionation of polar and non-polar compounds, respectively. The application of a single chromatographic stationary phase to a complex biological extract results in a significant proportion of compounds which elute in the non-retained fraction, where they are poorly detected because of a combination of ion suppression and the co-elution of isomeric compounds. Thus coverage of both polar and non-polar components of the metabolome generally involves multiple an..View full abstract
M McConville is an NHMRC Principal Research Fellow. U. Roessner is an ARC Future Fellow. The authors are grateful to the Victorian Node of Metabolomics Australia, which is funded through Bioplatforms Australia Pty Ltd, a National Collaborative Research Infrastructure Strategy (NCRIS), 5.1 biomolecular platforms and informatics investment, and co-investment from the Victorian State government and The University of Melbourne.