Journal article
Surface Engineering of Polypropylene Membranes with Carbonic Anhydrase-Loaded Mesoporous Silica Nanoparticles for Improved Carbon Dioxide Hydration
JKJ Yong, J Cui, KL Cho, GW Stevens, F Caruso, SE Kentish
Langmuir | Published : 2015
Abstract
Carbonic anhydrase (CA) is a native enzyme that facilitates the hydration of carbon dioxide into bicarbonate ions. This study reports the fabrication of thin films of active CA enzyme onto a porous membrane substrate using layer-by-layer (LbL) assembly. Deposition of multilayer films consisting of polyelectrolytes and CA was monitored by quartz crystal microgravimetry, while the enzymatic activity was assayed according to the rates of p-nitrophenylacetate (p-NPA) hydrolysis and CO2 hydration. The fabrication of the films onto a nonporous glass substrate showed CO2 hydration rates of 0.52 ± 0.09 μmol cm-2 min-1 per layer of bovine CA and 2.6 ± 0.7 μmol cm-2 min-1 per layer of a thermostable m..
View full abstractRelated Projects (1)
Grants
Funding Acknowledgements
The work was supported by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (Project number CE140100036) and the Particulate Fluids Processing Centre (PFPC), a Special Research Centre of the Australian Research Council. K.L.C. is the recipient of a John Stocker Postdoctoral Fellowship from the Science and Industry Endowment Fund. Novozymes A/S kindly supplied the enzyme, NCA. These sources of support are gratefully acknowledged.