Journal article
Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
Ian R Monk, Jai J Tree, Benjamin P Howden, Timothy P Stinear, Timothy J Foster
MBIO | AMER SOC MICROBIOLOGY | Published : 2015
Abstract
UNLABELLED: Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of S. aureus clonal complexes 1, 8, 30, and ST93. The IMXXB strains enable direct, high-efficiency transformation and streamlined genetic manipulation of major S. aureus lineages. IMPORTANCE: The genetic manipulation of clinical S. aureus isolates has been hampered due to the presence of restriction modi..
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Grants
Awarded by Science Foundation Ireland
Awarded by National Health and Medical Research Council, Australia
Funding Acknowledgements
We acknowledge financial support from Science Foundation Ireland (grant no. 08/IN.1/B1845) to TJF and National Health and Medical Research Council, Australia, fellowships to B.P.H. (APP1023526) and T.P.S. (APP1008549).