Journal article

Specific humoral response of hosts with variable schistosomiasis susceptibility

Patrick Driguez, Hamish EG McWilliam, Soraya Gaze, David Piedrafita, Mark S Pearson, Rie Nakajima, Mary Duke, Angela Trieu, Denise L Doolan, Fernanda C Cardoso, Algis Jasinskas, Geoffrey N Gobert, Philip L Felgner, Alex Loukas, Els Meeusen, Donald P McManus



The schistosome blood flukes are some of the largest global causes of parasitic morbidity. Further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. Therefore, for the purposes of antigen discovery, sera and antibody-secreting cell (ASC) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. Following Schistosoma japonicum infection, rats had reduced pathology, increased antibody responses and broader antigen recognition profiles compared with mice. With successive infections, rat global serological reactivity and the number of recognized antigens ..

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Funding Acknowledgements

We are grateful to Drs Andreas Hoffman, Asiah Osman, Wenbao Zhang and Jun Li for providing recombinant S. mansoni and S. japonicum proteins, and Dr Carla Proietti for critical review of the manuscript. DPM is a National Health and Medical Research Council (NHMRC) of Australia Senior Principal Research Fellow; AL and DLD are NHMRC Principal Research Fellows. This study received project and program grant support from NHMRC.