Journal article

EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance

Thao M Nguyen, Agnieszka Arthur, Romana Panagopoulos, Sharon Paton, John D Hayball, Andrew CW Zannettino, Louise E Purton, Koichi Matsuo, Stan Gronthos

STEM CELLS | WILEY | Published : 2015


The tyrosine kinase receptor, EphB4, mediates cross-talk between stromal and hematopoietic populations during bone remodeling, fracture repair and arthritis, through its interactions with the ligand, ephrin-B2. This study demonstrated that transgenic EphB4 mice (EphB4 Tg), over-expressing EphB4 under the control of collagen type-1 promoter, exhibited higher frequencies of osteogenic cells and hematopoietic stem/progenitor cells (HSC), correlating with a higher frequency of long-term culture-initiating cells (LTC-IC), compared with wild type (WT) mice. EphB4 Tg stromal feeder layers displayed a greater capacity to support LTC-IC in vitro, where blocking EphB4/ephrin-B2 interactions decreased ..

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Awarded by NHMRC Project

Funding Acknowledgements

We thank the staff at the Detmold Family Imaging Facility, Kate Pilkington and Rebecca Salmon, for their invaluable flow cytometric advice, the staff at the Radiation Oncology Department, Royal Adelaide Hospital, Josef-Binh Nguyen, for the use of Linac X-ray irradiator, Dr. Hayley Ramshaw at the SA Pathology, and Dr. Iain Comerford at the University of Adelaide for their invaluable advice on bone marrow transplantation. This work was supported by the University of South Australia Post graduate Award, Royal Adelaide Hospital Mary Overton Fellowship and NHMRC Project Grant #1023390.