Biomimetic Topography and Chemistry Control Cell Attachment to Amyloid Fibrils
Nicholas P Reynolds, Mirren Charnley, Marie N Bongiovanni, Patrick G Hartley, Sally L Gras
BIOMACROMOLECULES | AMER CHEMICAL SOC | Published : 2015
Networks of nanoscale fibrous coatings made from self-assembled peptides are promising candidates for biomaterials that can promote the growth of mammalian cells. One particularly attractive feature is the possibility of adding biofunctional sequences to peptides to promote cell attachment. We deconvolute the topographic and chemical effects of nanoscale fibrils on cells by depositing a plasma polymer film on TTR1-based fibrils decorated with a range of cell adhesive chemistries (RGD and cycloRGDfK), producing a surface that retains the nanoscale fibrous topography of surface-bound fibrils but lacks the fibril surface chemistry. The surface topography was found to influence cell toxicity and..View full abstract
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Awarded by Swiss National Science Foundation (SNSF)
Awarded by ARC Dairy Innovation Hub
Peptide synthesis of the TTR1-cycloRGDfK peptide was carried out by John Karas in the Peptide Technology Facility of the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne. N.P.R and M.C. acknowledge personal fellowships from the Swiss National Science Foundation (SNSF) (PBSKP2_145829/1 and P300P3_154664/1, respectively). N.P.R. also acknowledges the Australian Research Council (ARC) Training Centre for Biodevices. S.L.G. is supported by the ARC Dairy Innovation Hub (IH120100005).