Journal article
Polycomb repressive complex 2 component Suz12 is required for hematopoietic stem cell function and lymphopoiesis
SCW Lee, S Miller, C Hyland, M Kauppi, M Lebois, LD Rago, D Metcalf, SA Kinkel, EC Josefsson, ME Blewitt, IJ Majewski, WS Alexander
Blood | Published : 2015
Abstract
Polycomb repressive complex 2 (PRC2) is a chromatin modifier that regulates stem cells in embryonic and adult tissues. Loss-of-function studies of PRC2 components have been complicated by early embryonic dependence on PRC2 activity and the partial functional redundancy of enhancer of zeste homolog 1 (Ezh1) and enhancer of zeste homolog 2 (Ezh2), which encode the enzymatic component of PRC2. Here, we investigated the role of PRC2 in hematopoiesis by conditional deletion of suppressor of zeste 12 protein homolog (Suz12), a core component of PRC2. Complete loss of Suz12 resulted in failure of hematopoiesis, both in the embryo and the adult, with a loss of maintenance of hematopoietic stem cells..
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Awarded by Australian National Health and Medical Research Council
Funding Acknowledgements
This work was supported by program (1016647 and 575500) and project (1011663) grants, fellowships (W.S.A., I.J.M., M.E.B.), and Independent Research Institutes Infrastructure Support Scheme grant (361646) from the Australian National Health and Medical Research Council, fellowships from the Australia Research Council (M.E.B.), an Australian Postgraduate Award (S.C.W.L.), the Carden Fellowship (D.M.) of the Cancer Council, Victoria, the Australian Cancer Research Fund, and Victorian State Government Operational Infrastructure Support.