Journal article
Ambient fine particulate matter suppresses in vivo proliferation of bone marrow stem cells through reactive oxygen species formation
Y Cui, F Jia, J He, X Xie, Z Li, M Fu, H Hao, Y Liu, DZ Liu, PJ Cowan, H Zhu, Q Sun, Z Liu
Plos One | Published : 2015
Abstract
Aims: Some environmental insults, such as fine particulate matter (PM) exposure, significantly impair the function of stem cells. However, it is unknown if PM exposure could affect the population of bone marrow stem cells (BMSCs). The present study was to investigate the effects of PM on BMSCs population and related mechanism(s). Main Metheods: PM was intranasally distilled into male C57BL/6 mice for one month. Flow cytometry with antibodies for BMSCs, Annexin V and BrdU ware used to determine the number of BMSCs and the levels of their apoptosis and proliferation in vivo. Phosphorylated Akt (P-Akt) level was determined in the BM cells with western blotting. Intracellular reactive oxygen spe..
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Awarded by National Institute of Environmental Health Sciences
Funding Acknowledgements
US NIH grant to Zhenguo Liu (NIH R01 HL094650) for experiment design and American Heart Association grant to HZ (AHA 12SDG12070174) for experiment design.