Immunoreactive localisation of P2Y1 receptors within the rat and human nodose ganglia and rat brainstem: Comparison with [α33P]deoxyadenosine 5′-triphosphate autoradiography

Abstract

The present study employed standard peroxidase immunohistochemistry to map the distribution of P2Y1 receptors in the rat brainstem and nodose ganglia and characterised the binding profile of [α33P]dATP. Binding of [α33P]dATP was fully displaceable by adenosine 5′-triphosphate (ATP), and was found on both human and rat nodose ganglia, and throughout the rat brainstem, including the nucleus tractus solitarius and ventrolateral medulla. [α33P]dATP binding in the human nodose ganglia was significantly displaced by both 2-methylthio ATP and α,β-methylene ATP, but not by uridine 5′-triphosphate, pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid, 8,8′-(carbonylbis(imino-4,1-phenylenecarbonylimin..