Characterisation of vasopressin V(1A), angiotensin AT1 and AT2 receptor distribution and density in normotensive and hypertensive rat brain stem and kidney: Effects of restraint stress

Abstract

In the present study, we have examined neurochemical correlates that may be involved in the differential cardiovascular responses observed in normotensive and hypertensive rats during stress. Using a restraint stress paradigm, both normotensive Wistar Kyoto (WKY) and Spontaneously Hypertensive rats (SHR) underwent acute (1 h restraint in a perspex tube), chronic (1 h restraint for ten consecutive days) or no restraint (control) stress. Following cessation of restraint, rats were processed by incubating sections of brain stem and kidney with [125I]-HO-LVA (0.03 nM) or [125I]Sar1Ile8-AngiotensinII (0.5 nM), in the presence of PD123319 (10 μM) or losartan (10 μM), to determine the distribution ..