Journal article

Visualisation of AMPA binding sites in the brain of mice lacking the adenosine A(2a) receptor

BJ Snell, JL Short, J Drago, C Ledent, AJ Lawrence

NEUROSCIENCE LETTERS | ELSEVIER SCI IRELAND LTD | Published : 2000

Abstract

Adenosine A(2a) receptor knockout mice (A(2a)R k/o) have been characterised as hypertensive, aggressive, anxious and hypoalgesic [12]. Thus, the present study was designed to investigate markers of glutamatergic transmission in specific brain nuclei associated with autonomic regulation. Visualisation of alpha-amino-3-hydroxy-5-methylisoxasole-4-propionic acid binding sites in the brains of A(2a)R k/o mice was achieved by utilising quantitative receptor autoradiography with (S)-[(3)H]-5-fluorowillardiine ([(3)H]FW:10 nM) on slide-mounted sections of mouse-brain. [(3)H]FW binding significantly increased in the nucleus tractus solitarius and area postrema of A(2a)R k/o mice compared with wildty..

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