Journal article
Regional distribution of low affinity kainate receptors in brain of Macaca fascicularis determined by autoradiography using [H-3](2S,4R)-4-methylglutamate
FY Carroll, DI Finkelstein, MK Horne, AJ Lawrence, D Crawford, G Paxinos, PM Beart
NEUROSCIENCE LETTERS | ELSEVIER SCI IRELAND LTD | Published : 1998
Abstract
Binding of [3H](2S,4R)-4-methylglutamate, a novel low affinity kainate receptor agonist, was studied in brain sections of a Macaca fascicularis monkey. In cerebellar sections, [3H](2S,4R)-4-methylglutamate bound to a single population of sites (KD = 20 nM) and was inhibited by various glutamate receptor ligands: kainate > 6-cyano-7-nitroquinoxaline-2,3-dione > L-glutamate >> AMPA. (S)-5-lodowillardiine and (RS)-2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl)propanoic acid (ATPA), drugs selective for the GluR5 subunit, displaced 50% and 40% of binding, respectively. Autoradiography revealed topographic binding of [3H](2S,4R)-4-methylglutamate. Binding in cortex was highest in layer 5 and rest..
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