Effects of acute ethanol on GABA release and GABA(A) receptor density in the rat mesolimbic system

Abstract

The present study has addressed whether acute ethanol administration mediates changes in GABA release and GABA(A) receptor density in the rat mesolimbic system. In vivo microdialysis was performed in the ventral pallidum and between the ventral tegmental area and substantia nigra of conscious ethanol-naive rats. Extracellular levels of endogenous GABA were stable in both brain regions and not significantly affected following administration of 5 ml of 20% ethanol by gavage, despite clear overt behavioral signs of intoxication. Two hours following ethanol administration, animals were decapitated and the brains processed for autoradiography. Adjacent tissue sections were incubated with [3H]SR95..