Journal article

NITRIC-OXIDE INCREASES INTERSTITIAL EXCITATORY AMINO-ACID RELEASE IN THE RAT DORSOMEDIAL MEDULLA-OBLONGATA

AJ LAWRENCE, B JARROTT

NEUROSCIENCE LETTERS | ELSEVIER SCI IRELAND LTD | Published : 1993

Abstract

In vivo microdialysis was employed to measure release of the neuroactive amino acids L-glutamate (Glu) and L-aspartate (Asp) in the dorsomedial medulla oblongata of the anaesthetised rat. Basal levels of endogenous extracellular Glu and Asp were increased over 5-fold and 3-fold, respectively, following perfusion with a depolarising stimulus of KCl. Intracerebral administration of the nitric oxide (NO) donor S-nitroso-N-acetylpenicillamine (SNAP, 30 microM) caused a 5-fold increase in extracellular Asp and a 2-fold increase in extracellular Glu, which was blocked by Methylene blue. These data suggest that NO, acting through guanylate cyclase, can affect excitatory amino acid neurotransmission..

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