The MUC1 mucin protects against Helicobacter pylori pathogenesis in mice by regulation of the NLRP3 inflammasome
Garrett Z Ng, Trevelyan R Menheniott, Alison L Every, Andrew Stent, Louise M Judd, Yok Teng Chionh, Poshmaal Dhar, Jasper C Komen, Andrew S Giraud, Timothy C Wang, Michael A McGuckin, Philip Sutton
GUT | BMJ PUBLISHING GROUP | Published : 2016
OBJECTIVES: The mucin MUC1, best known for providing an epithelial barrier, is an important protective host factor in both humans and mice during Helicobacter pylori pathogenesis. This study aimed to identify the long-term consequences of MUC1 deficiency on H. pylori pathogenesis and the mechanism by which MUC1 protects against H. pylori gastritis. DESIGN: Wildtype and Muc1(-/-) mice were infected for up to 9 months, and the gastric pathology, immunological response and epigenetic changes assessed. The effects of MUC1 on the inflammasome, a potent inflammatory pathway, were examined in macrophages and H. pylori-infected mice deficient in both MUC1 and inflammasome components. RESULTS: Muc1(-..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
This work was supported by the Victorian Government's Operational Infrastructure Support Program and by Project Grant #1046254 from the National Health and Medical Research Council (NHMRC) of Australia. PS and MAM are supported by Senior Research Fellowships from the NHMRC and GZN was partly supported by a Melbourne Research Scholarship from the University of Melbourne.