Journal article

Functional Heterogeneity and Antimycobacterial Effects of Mouse Mucosal-Associated Invariant T Cells Specific for Riboflavin Metabolites

Isaac G Sakala, Lars Kjer-Nielsen, Christopher S Eickhoff, Xiaoli Wang, Azra Blazevic, Ligong Liu, David P Fairlie, Jamie Rossjohn, James McCluskey, Daved H Fremont, Ted H Hansen, Daniel F Hoft

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2015

Abstract

Mucosal-associated invariant T (MAIT) cells have a semi-invariant TCR Vα-chain, and their optimal development is dependent upon commensal flora and expression of the nonpolymorphic MHC class I-like molecule MR1. MAIT cells are activated in an MR1-restricted manner by diverse strains of bacteria and yeast, suggesting a widely shared Ag. Recently, human and mouse MR1 were found to bind bacterial riboflavin metabolites (ribityllumazine [RL] Ags) capable of activating MAIT cells. In this study, we used MR1/RL tetramers to study MR1 dependency, subset heterogeneity, and protective effector functions important for tuberculosis immunity. Although tetramer(+) cells were detected in both MR1(+/+) and..

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University of Melbourne Researchers

Grants

Awarded by National Institutes of Health Grants


Awarded by National Health and Medical Research Council of Australia Program Grant


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by National Institutes of Health Grants R01-AI046553 (to T.H.H.) and R01-A148391 (to D.F.H), a National Health and Medical Research Council of Australia Senior Principal Research Fellowship (to D.P.F.), a National Health and Medical Research Council of Australia Fellowship (to J.R.), and National Health and Medical Research Council of Australia Program Grant 1016629 (to J.R. and J.M.).