Coaggregation of Candida albicans, Actinomyces naeslundii and Streptococcus mutans is Candida albicans strain dependent
Mohd Hafiz Arzmi, Stuart Dashper, Deanne Catmull, Nicola Cirillo, Eric C Reynolds, Michael McCullough
FEMS YEAST RESEARCH | OXFORD UNIV PRESS | Published : 2015
Microbial interactions are necessarily associated with the development of polymicrobial oral biofilms. The objective of this study was to determine the coaggregation of eight strains of Candida albicans with Actinomyces naeslundii and Streptococcus mutans. In autoaggregation assays, C. albicans strains were grown in RPMI-1640 and artificial saliva medium (ASM) whereas bacteria were grown in heart infusion broth. C. albicans, A. naeslundii and S. mutans were suspended to give 10(6), 10(7) and 10(8) cells mL(-1) respectively, in coaggregation buffer followed by a 1 h incubation. The absorbance difference at 620 nm (ΔAbs) between 0 h and 1 h was recorded. To study coaggregation, the same protoc..View full abstract
This work was funded by Oral Health Cooperative Research Centre (OHCRC) and the Melbourne Dental School.