Identification of phenotypically and functionally heterogeneous mouse mucosal-associated invariant T cells using MR1 tetramers

Azad Rahimpour; Hui Fern Koay; Anselm Enders; Rhiannon Clanchy; Sidonia BG Eckle; Bronwyn Meehan; Zhenjun Chen; Belinda Whittle; Ligong Liu; David P Fairlie; Chris C Goodnow; James McCluskey; Jamie Rossjohn; Adam P Uldrich; Daniel G Pellicci; Dale I Godfrey

Journal article

Identification of phenotypically and functionally heterogeneous mouse mucosal-associated invariant T cells using MR1 tetramers

Azad Rahimpour, Hui Fern Koay, Anselm Enders, Rhiannon Clanchy, Sidonia BG Eckle, Bronwyn Meehan, Zhenjun Chen, Belinda Whittle, Ligong Liu, David P Fairlie, Chris C Goodnow, James McCluskey, Jamie Rossjohn, Adam P Uldrich, Daniel G Pellicci, Dale I Godfrey

JOURNAL OF EXPERIMENTAL MEDICINE | ROCKEFELLER UNIV PRESS | Published : 2015

DOI: 10.1084/jem.20142110

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Abstract

Studies on the biology of mucosal-associated invariant T cells (MAIT cells) in mice have been hampered by a lack of specific reagents. Using MR1-antigen (Ag) tetramers that specifically bind to the MR1-restricted MAIT T cell receptors (TCRs), we demonstrate that MAIT cells are detectable in a broad range of tissues in C57BL/6 and BALB/c mice. These cells include CD4(-)CD8(-), CD4(-)CD8(+), and CD4(+)CD8(-) subsets, and their frequency varies in a tissue- and strain-specific manner. Mouse MAIT cells have a CD44(hi)CD62L(lo) memory phenotype and produce high levels of IL-17A, whereas other cytokines, including IFN-γ, IL-4, IL-10, IL-13, and GM-CSF, are produced at low to moderate levels. Consi..

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University of Melbourne Researchers

Dale Godfrey Author Microbiology and Immunology

Fern Koay Author Microbiology and Immunology

Adam Uldrich Author Microbiology and Immunology

Daniel Pellicci Author Microbiology and Immunology

James McCluskey Author

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Funding Acknowledgements

This work was supported by a project grant and program grants from the National Health and Medical Research Council of Australia (NHMRC; 108394, 1013667, and 1016629) and the Australian Research Council (ARC; CE140100011 and LE110100106). A. Enders is supported by an NHMRC Career Development Fellowship (1035858); D.G. Pellicci is supported by an NHMRC Early Career Fellowship (1054431); A.P. Uldrich is supported by an ARC Future Fellowship (FT140100278); D.I. Godfrey and D.P. Fairlie are supported by NHMRC Senior Principal Research Fellowships (1020770 and 1027369); J. Rossjohn is supported by an NHMRC Australia Fellowship (AF50).