Journal article
Transient receptor potential cation channel subfamily v member 1 expressing corneal sensory neurons can be subdivided into at least three subpopulations
A Alamri, R Bron, JA Brock, JJ Ivanusic
Frontiers in Neuroanatomy | Published : 2015
Abstract
The cornea is innervated by three main functional classes of sensory neurons: polymodal nociceptors, pure mechano-nociceptors and cold-sensing neurons. Here we explored transient receptor potential cation channel subfamily V member 1 (TRPV1) expression in guinea pig corneal sensory neurons, a widely used molecular marker of polymodal nociceptors. We used retrograde tracing to identify corneal afferent neurons in the trigeminal ganglion (TG) and double label in situ hybridization and/or immunohistochemistry to determine their molecular profile. In addition, we used immunohistochemistry to reveal the neurochemistry and structure of TRPV1 expressing nerve endings in the corneal epithelium. Appr..
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Funding Acknowledgements
This work was supported by a Development grant from the Department of Anatomy and Neuroscience, University of Melbourne. AA was supported by a scholarship provided by Taif University/Ministry of Education, Saudi Arabia. Access to the slide scanner was provided by the Histopathology and Organ Pathology Service at the University of Melbourne. Access to confocal imaging was provided by the Biological and Optical Microscopy Platform at the University of Melbourne.