Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes
Greg Tesch, Karly C Sourris, Shaun A Summers, Domenica McCarthy, Micheal S Ward, Danielle J Borg, Linda A Gallo, Amelia K Fotheringham, Allison R Pettit, Felicia YT Yap, Brooke E Harcourt, Adeline LY Tan, Joshua Y Kausman, David Nikolic-Paterson, Arthur R Kitching, Josephine M Forbes
DIABETOLOGIA | SPRINGER | Published : 2014
AIMS/HYPOTHESIS: The AGEs and the receptor for AGEs (RAGE) are known contributors to diabetic complications. RAGE also has a physiological role in innate and adaptive immunity and is expressed on immune cells. The aim of this study was to determine whether deletion of RAGE from bone-marrow-derived cells influences the pathogenesis of experimental diabetic nephropathy. METHODS: Groups (n = 8/group) of lethally irradiated 8 week old wild-type (WT) mice were reconstituted with bone marrow from WT (WT → WT) or RAGE-deficient (RG) mice (RG → WT). Diabetes was induced using multiple low doses of streptozotocin after 8 weeks of bone marrow reconstitution and mice were followed for a further 24 week..View full abstract
JMF is supported by a Senior Research Fellowship from the National Health and Medical Research Council of Australia (NHMRC). LAG and MSW are supported by research fellowships from the Heart Foundation Australia and JDRF International, respectively. FYTY and AKF were supported by PhD scholarships from NHMRC and the University of Queensland, QLD, Australia. This research was supported by the NHMRC and JDRF International. The funding bodies (NHMRC and JDRF) had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.