Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis
Jessie M Sutherland, Alexander P Sobinoff, Kara M Gunter, Barbara A Fraser, Victoria Pye, Ilana R Bernstein, Evan Boon, Nicole A Siddall, Luisa I De Andres, Gary R Hime, Janet E Holt, Thomas Graf, Eileen A McLaughlin
BIOMOLECULES | MDPI | Published : 2015
Characterizing the mechanisms underlying follicle development in the ovary is crucial to understanding female fertility and is an area of increasing research interest. The RNA binding protein Musashi is essential for post-transcriptional regulation of oocyte maturation in Xenopus and is expressed during ovarian development in Drosophila. In mammals Musashi is important for spermatogenesis and male fertility, but its role in the ovary has yet to be characterized. In this study we determined the expression of mammalian Musashi proteins MSI1 and MSI2 during mouse folliculogenesis, and through the use of a MSI2-specific knockout mouse model we identified that MSI2 is essential for normal follicl..View full abstract
Financial assistance provided to Eileen A. McLaughlin and Gary R. Hime by the Australian Research Council, National Health and Medical Research Council, and Hunter Medical Research Institute. Additional support was provided the ARC Centre of Excellence in Biotechnology (Eileen A. McLaughlin and Gary R. Hime). Jessie M. Sutherland, Alexander P. Sobinoff, and Kara M. Gunter were recipients of Australian Postgraduate Award PhD scholarships. Janet E. Holt was a recipient of an ARC Discovery Early Career Researcher Award.