Journal article

Inflammation-induced abnormalities in the subcellular localization and trafficking of the neurokinin 1 receptor in the enteric nervous system

Daniel P Poole, TinaMarie Lieu, Juan Carlos Pelayo, Emily M Eriksson, Nicholas A Veldhuis, Nigel W Bunnett

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2015

Abstract

Activated G protein-coupled receptors traffic to endosomes and are sorted to recycling or degradative pathways. Endosomes are also a site of receptor signaling of sustained and pathophysiologically important processes, including inflammation. However, the mechanisms of endosomal sorting of receptors and the impact of disease on trafficking have not been fully defined. We examined the effects of inflammation on the subcellular distribution and trafficking of the substance P (SP) neurokinin 1 receptor (NK1R) in enteric neurons. We studied NK1R trafficking in enteric neurons of the mouse colon using immunofluorescence and confocal microscopy. The impact of inflammation was studied in IL10(-/-)-..

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Grants

Awarded by National Institute of Diabetes and Digestive and Kidney Diseases


Awarded by National Health and Medical Research Council Australia


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Funding Acknowledgements

This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grant DK-39957 (N. W. Bunnett), National Health and Medical Research Council Australia Grants 633033 and 108305 (N. W. Bunnett) and 454858 and 1049730 (D. P. Poole), the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (N. W. Bunnett), and Monash University (N. W. Bunnett).