Journal article
Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease
TR Elliott, NB Rayment, BN Hudspith, RE Hands, K Taylor, GC Parkes, NJ Prescott, L Petrovska, J Hermon-Taylor, J Brostoff, A Boussioutas, CG Mathew, SA Bustin, JD Sanderson
BMC Gastroenterology | BMC | Published : 2015
Abstract
Background: Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn's disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods: Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results: E. coli-laden LP macrophages were identified commonly in muco..
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Funding Acknowledgements
We acknowledge the National Institute for Health Research (NIHR) Biomedical Research Centre award to Guy's & St. Thomas' National Health Service (NHS) Trust, King's College London. We also acknowledge the Broad Foundation, USA who contributed funding to this work by an Inflammatory Bowel Disease grant. N.B.R was funded by the Broad Foundation and B.N.H & G.C.P were funded by Foundation for Allergy Information and Research, UK. The authors would like to thank Professor Anthony d'Apice and Professor Peter Cowan, Immunology Research Centre, St Vincent's Hospital, Melbourne, Australia, for their critical review of this manuscript.